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2 nbdg fluorescent glucose probe  (MedChemExpress)


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    MedChemExpress 2 nbdg fluorescent glucose probe
    Regulation of SARS-CoV-2 S protein expression on mitochondrial quality control and metabolic reprogramming in lung epithelial cells. (A-F, H-L) BEAS-2B cells were transfected with pCMV3-SARS-CoV-2 S (pCMV-S, 1 μg for 24 h) to induce the S protein expression. ( A) TEM observation of mitochondrial morphology in cells. N, nucleus. M, mitochondria. Scale bar, 5 μm for the gobal view and 2 μm for the zoom view. ( B) IF assay of MitoTracker (green) and LysoTracker (red) was used to assess the intensity and co-localization in cells. Scale bar, 10 μm. ( C) WB analysis of mitochondrial dynamics-associated proteins (Drp1, p-Drp1 Ser616 , and Mfn2). ( D) Flow cytometry (FCM) assay of mitochondrial ROS levels using MitoSOX (red) in cells. ( E) WB analysis of mitochondrial damage-related proteins (Bax, Bcl2, and Cyto C). ( F) JC-1 assay of mitochondrial membrane potential. ( G) The GEO dataset ( GSE147507 ) from SARS-CoV-2-infected A549 cell samples (n = 6) was screened, while mock samples (n = 5) used as controls. The heatmap depicts the DEGs of glycolysis-related genes. ( H) Relative mRNA levels of mitochondrial energy metabolism-related genes in cells. ( I) FCM assay of glucose flux using fluorescent glucose <t>analog</t> <t>2-NBDG</t> in cells. ( J) Detection of ATP content in cells. ( K) WB analysis of glycolysis-related proteins (HIF1α, HK2, and PKM2). ( L) Levels of glycolysis rate-limiting enzymes (hexokinase and pyruvate kinase) activities and glycolysis product lactic acid. (M−O) BEAS-2B cells were pre-treated with the hexokinase inhibitor 2-deoxy-D-glucose (2-DG, 20 μM for 3 h), followed by transfection with pCMV-S (1 μg for 24 h) or mock transfection. (M) WB analysis of NLRP3 inflammasome activation (NLRP3, ASC, and p10) and pyroptosis-related proteins (GSDMD and IL-1β). ( N) LDH release assay and ELISA detection of IL-1β release levels in the supernatant. ( O) Cell viability was measured using the MTT assay. *, P < 0.05, compare to the control group or corresponding group. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    2 Nbdg Fluorescent Glucose Probe, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 98 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2 nbdg fluorescent glucose probe/product/MedChemExpress
    Average 95 stars, based on 98 article reviews
    2 nbdg fluorescent glucose probe - by Bioz Stars, 2026-03
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    Images

    1) Product Images from "Palmitoylated COX-2 Cys555 reprogrammed mitochondrial metabolism in pyroptotic inflammatory injury in patients with post-acute COVID-19 syndrome"

    Article Title: Palmitoylated COX-2 Cys555 reprogrammed mitochondrial metabolism in pyroptotic inflammatory injury in patients with post-acute COVID-19 syndrome

    Journal: Journal of Advanced Research

    doi: 10.1016/j.jare.2025.05.005

    Regulation of SARS-CoV-2 S protein expression on mitochondrial quality control and metabolic reprogramming in lung epithelial cells. (A-F, H-L) BEAS-2B cells were transfected with pCMV3-SARS-CoV-2 S (pCMV-S, 1 μg for 24 h) to induce the S protein expression. ( A) TEM observation of mitochondrial morphology in cells. N, nucleus. M, mitochondria. Scale bar, 5 μm for the gobal view and 2 μm for the zoom view. ( B) IF assay of MitoTracker (green) and LysoTracker (red) was used to assess the intensity and co-localization in cells. Scale bar, 10 μm. ( C) WB analysis of mitochondrial dynamics-associated proteins (Drp1, p-Drp1 Ser616 , and Mfn2). ( D) Flow cytometry (FCM) assay of mitochondrial ROS levels using MitoSOX (red) in cells. ( E) WB analysis of mitochondrial damage-related proteins (Bax, Bcl2, and Cyto C). ( F) JC-1 assay of mitochondrial membrane potential. ( G) The GEO dataset ( GSE147507 ) from SARS-CoV-2-infected A549 cell samples (n = 6) was screened, while mock samples (n = 5) used as controls. The heatmap depicts the DEGs of glycolysis-related genes. ( H) Relative mRNA levels of mitochondrial energy metabolism-related genes in cells. ( I) FCM assay of glucose flux using fluorescent glucose analog 2-NBDG in cells. ( J) Detection of ATP content in cells. ( K) WB analysis of glycolysis-related proteins (HIF1α, HK2, and PKM2). ( L) Levels of glycolysis rate-limiting enzymes (hexokinase and pyruvate kinase) activities and glycolysis product lactic acid. (M−O) BEAS-2B cells were pre-treated with the hexokinase inhibitor 2-deoxy-D-glucose (2-DG, 20 μM for 3 h), followed by transfection with pCMV-S (1 μg for 24 h) or mock transfection. (M) WB analysis of NLRP3 inflammasome activation (NLRP3, ASC, and p10) and pyroptosis-related proteins (GSDMD and IL-1β). ( N) LDH release assay and ELISA detection of IL-1β release levels in the supernatant. ( O) Cell viability was measured using the MTT assay. *, P < 0.05, compare to the control group or corresponding group. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Figure Legend Snippet: Regulation of SARS-CoV-2 S protein expression on mitochondrial quality control and metabolic reprogramming in lung epithelial cells. (A-F, H-L) BEAS-2B cells were transfected with pCMV3-SARS-CoV-2 S (pCMV-S, 1 μg for 24 h) to induce the S protein expression. ( A) TEM observation of mitochondrial morphology in cells. N, nucleus. M, mitochondria. Scale bar, 5 μm for the gobal view and 2 μm for the zoom view. ( B) IF assay of MitoTracker (green) and LysoTracker (red) was used to assess the intensity and co-localization in cells. Scale bar, 10 μm. ( C) WB analysis of mitochondrial dynamics-associated proteins (Drp1, p-Drp1 Ser616 , and Mfn2). ( D) Flow cytometry (FCM) assay of mitochondrial ROS levels using MitoSOX (red) in cells. ( E) WB analysis of mitochondrial damage-related proteins (Bax, Bcl2, and Cyto C). ( F) JC-1 assay of mitochondrial membrane potential. ( G) The GEO dataset ( GSE147507 ) from SARS-CoV-2-infected A549 cell samples (n = 6) was screened, while mock samples (n = 5) used as controls. The heatmap depicts the DEGs of glycolysis-related genes. ( H) Relative mRNA levels of mitochondrial energy metabolism-related genes in cells. ( I) FCM assay of glucose flux using fluorescent glucose analog 2-NBDG in cells. ( J) Detection of ATP content in cells. ( K) WB analysis of glycolysis-related proteins (HIF1α, HK2, and PKM2). ( L) Levels of glycolysis rate-limiting enzymes (hexokinase and pyruvate kinase) activities and glycolysis product lactic acid. (M−O) BEAS-2B cells were pre-treated with the hexokinase inhibitor 2-deoxy-D-glucose (2-DG, 20 μM for 3 h), followed by transfection with pCMV-S (1 μg for 24 h) or mock transfection. (M) WB analysis of NLRP3 inflammasome activation (NLRP3, ASC, and p10) and pyroptosis-related proteins (GSDMD and IL-1β). ( N) LDH release assay and ELISA detection of IL-1β release levels in the supernatant. ( O) Cell viability was measured using the MTT assay. *, P < 0.05, compare to the control group or corresponding group. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Techniques Used: Expressing, Control, Transfection, Flow Cytometry, Membrane, Infection, Activation Assay, Lactate Dehydrogenase Assay, Enzyme-linked Immunosorbent Assay, MTT Assay



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    Regulation of SARS-CoV-2 S protein expression on mitochondrial quality control and metabolic reprogramming in lung epithelial cells. (A-F, H-L) BEAS-2B cells were transfected with pCMV3-SARS-CoV-2 S (pCMV-S, 1 μg for 24 h) to induce the S protein expression. ( A) TEM observation of mitochondrial morphology in cells. N, nucleus. M, mitochondria. Scale bar, 5 μm for the gobal view and 2 μm for the zoom view. ( B) IF assay of MitoTracker (green) and LysoTracker (red) was used to assess the intensity and co-localization in cells. Scale bar, 10 μm. ( C) WB analysis of mitochondrial dynamics-associated proteins (Drp1, p-Drp1 Ser616 , and Mfn2). ( D) Flow cytometry (FCM) assay of mitochondrial ROS levels using MitoSOX (red) in cells. ( E) WB analysis of mitochondrial damage-related proteins (Bax, Bcl2, and Cyto C). ( F) JC-1 assay of mitochondrial membrane potential. ( G) The GEO dataset ( GSE147507 ) from SARS-CoV-2-infected A549 cell samples (n = 6) was screened, while mock samples (n = 5) used as controls. The heatmap depicts the DEGs of glycolysis-related genes. ( H) Relative mRNA levels of mitochondrial energy metabolism-related genes in cells. ( I) FCM assay of glucose flux using fluorescent glucose analog 2-NBDG in cells. ( J) Detection of ATP content in cells. ( K) WB analysis of glycolysis-related proteins (HIF1α, HK2, and PKM2). ( L) Levels of glycolysis rate-limiting enzymes (hexokinase and pyruvate kinase) activities and glycolysis product lactic acid. (M−O) BEAS-2B cells were pre-treated with the hexokinase inhibitor 2-deoxy-D-glucose (2-DG, 20 μM for 3 h), followed by transfection with pCMV-S (1 μg for 24 h) or mock transfection. (M) WB analysis of NLRP3 inflammasome activation (NLRP3, ASC, and p10) and pyroptosis-related proteins (GSDMD and IL-1β). ( N) LDH release assay and ELISA detection of IL-1β release levels in the supernatant. ( O) Cell viability was measured using the MTT assay. *, P < 0.05, compare to the control group or corresponding group. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Journal: Journal of Advanced Research

    Article Title: Palmitoylated COX-2 Cys555 reprogrammed mitochondrial metabolism in pyroptotic inflammatory injury in patients with post-acute COVID-19 syndrome

    doi: 10.1016/j.jare.2025.05.005

    Figure Lengend Snippet: Regulation of SARS-CoV-2 S protein expression on mitochondrial quality control and metabolic reprogramming in lung epithelial cells. (A-F, H-L) BEAS-2B cells were transfected with pCMV3-SARS-CoV-2 S (pCMV-S, 1 μg for 24 h) to induce the S protein expression. ( A) TEM observation of mitochondrial morphology in cells. N, nucleus. M, mitochondria. Scale bar, 5 μm for the gobal view and 2 μm for the zoom view. ( B) IF assay of MitoTracker (green) and LysoTracker (red) was used to assess the intensity and co-localization in cells. Scale bar, 10 μm. ( C) WB analysis of mitochondrial dynamics-associated proteins (Drp1, p-Drp1 Ser616 , and Mfn2). ( D) Flow cytometry (FCM) assay of mitochondrial ROS levels using MitoSOX (red) in cells. ( E) WB analysis of mitochondrial damage-related proteins (Bax, Bcl2, and Cyto C). ( F) JC-1 assay of mitochondrial membrane potential. ( G) The GEO dataset ( GSE147507 ) from SARS-CoV-2-infected A549 cell samples (n = 6) was screened, while mock samples (n = 5) used as controls. The heatmap depicts the DEGs of glycolysis-related genes. ( H) Relative mRNA levels of mitochondrial energy metabolism-related genes in cells. ( I) FCM assay of glucose flux using fluorescent glucose analog 2-NBDG in cells. ( J) Detection of ATP content in cells. ( K) WB analysis of glycolysis-related proteins (HIF1α, HK2, and PKM2). ( L) Levels of glycolysis rate-limiting enzymes (hexokinase and pyruvate kinase) activities and glycolysis product lactic acid. (M−O) BEAS-2B cells were pre-treated with the hexokinase inhibitor 2-deoxy-D-glucose (2-DG, 20 μM for 3 h), followed by transfection with pCMV-S (1 μg for 24 h) or mock transfection. (M) WB analysis of NLRP3 inflammasome activation (NLRP3, ASC, and p10) and pyroptosis-related proteins (GSDMD and IL-1β). ( N) LDH release assay and ELISA detection of IL-1β release levels in the supernatant. ( O) Cell viability was measured using the MTT assay. *, P < 0.05, compare to the control group or corresponding group. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Article Snippet: The impact of SARS-CoV-2 S protein expression on glucose uptake in lung epithelial cells was evaluated using the 2-NBDG fluorescent glucose probe (MedChemExpress, Monmouth Junction, NJ, USA).

    Techniques: Expressing, Control, Transfection, Flow Cytometry, Membrane, Infection, Activation Assay, Lactate Dehydrogenase Assay, Enzyme-linked Immunosorbent Assay, MTT Assay